منابع مشابه
Localized Secretion of Acid Phosphatase
Secretion of cell wall-bound acid phosphatase by Saccharomyces cerevisiae occurs along a restricted portion of the cell surface. Acid phosphatase activity produced during derepressed synthesis on a phosphate-limited growth medium is detected with an enzymespecific stain and is localized initially to the bud portion of a dividing cell . After two to three generations of phosphate-limited growth,...
متن کاملSoybean root nodule acid phosphatase.
Acid phosphatases are ubiquitous enzymes that exhibit activity against a variety of substrates in vitro, although little is known about their intracellular function. In this study, we report the isolation, characterization, and partial sequence of the major acid phosphatase from soybean (Glycine max L.) root nodules. The phosphatase was purified predominantly as a heterodimer with subunits of 2...
متن کاملAutomated determination of acid phosphatase.
A procedureis presentedfor the automated determination of acid phosphatase activity in biological materials from either plant or animal origin. The Technicon N-i flow diagram has been simplified, and the reagents for the measurement of enzymatically produced phenolic substanceshave been modified without lossof range or sensitivity. Both phenyiphosphateand a-naphthylphosphate, introduced by Babs...
متن کاملKinetic Properties of Acid Phosphatase
Introduction Phosphatases are enzymes that remove phosphate groups from substrates. Phosphorylated compounds are widely distributed in living systems. They serve as storage forms for energy (e.g., ATP and phosphocreatine), as components of informational macromolecules (i.e., nucleotides and deoxynucleotides), as allosteric effectors of certain enzymes (e.g., fructose-1-6bisphosphate), and as se...
متن کاملIntracellular Localization of Acid Phosphatase
The acid phosphatase activity of rat liver homogenates and of nuclear and cytoplasmic fractions isolated therefrom (by differential centrifugation) was determined biochemically in a series of experiments. For each liver, Gomori's histochemical test for acid phosphatase was run in parallel. No correlation was found between the biochemical and the histochemical results. According to the former, t...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1957
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64955-5